Lipids, especially phosphoinositides, play multiple roles in cell function. Metabolic modification of these lipids can determine cell fate with the lowest abundance lipids often having the greatest impact. We specialize in the measurement of difficult to detect and low abundance lipids such as phosphoinositides, sphingolipids, phosphatidic acid, lysolipids, diacylglyerols and more.
(We accept credit payments for Completed Services - See Link below)
Services
Analytics
We analyze phosphoinositides and other lipids in extracts from a range of sources spanning tissue samples, cell cultures, bacteria and viruses. Available services include lipid extraction, derivatization and qualitative or quantitative lipidomic measurements via LC/MS/MS or TOF with data analysis tailored to your needs.
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InnovationUnusual sample or target lipid? We do method development. Would you like to set up lipidomics capabilities in your lab? We can help.
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DiscoveryWe provide customized solutions to your Lipidomics analysis challenges. See publications from some of our collaborators and clients below.
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Publications - Collaborators and Clients
- Yeh YT, Sona C, Yan X, Li Y, Pathak A, McDermott MI, Xie Z, Liu L, Arunagiri A, Wang Y, Cazenave-Gassiot A, Ghosh A, von Meyenn F, Kumarasamy S, Najjar SM, Jia S, Wenk MR, Traynor-Kaplan A, Arvan P, Barg S, Bankaitis VA, Poy MN. Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction. (2023) Nat Commun. Jul 17;14(1):4250. doi: 10.1038/s41467-023-39978-1. PMID: 37460527; PMCID: PMC10352338.
- Yildirim AD, Citir M, Dogan AE, Veli Z, Yildirim Z, Tufanli O, Traynor-Kaplan A, Schultz C, Erbay E. ER Stress-Induced Sphingosine-1-Phosphate Lyase Phosphorylation Potentiates the Mitochondrial Unfolded Protein Response. J Lipid Res. 2022 Oct;63(10):100279. doi: 10.1016/j.jlr.2022.100279. Epub 2022 Sep 12. PMID: 36100091; PMCID: PMC9579414.
- Citir M, Müller R, Hauke S, Traynor-Kaplan A, Schultz C. Monitoring the cellular metabolism of a membrane-permeant photo-caged phosphatidylinositol 3,4,5-trisphosphate derivative. (2021) Chem Phys Lipids. Nov;241:105124. doi: 10.1016/j.chemphyslip.2021.105124. Epub 2021 Sep 9. PMID: 34509466.
- Hamid SM, Citir M, Terzi EM, Cimen I, Yildirim Z, Dogan AE, Kocaturk B, Onat UI, Arditi M, Weber C, Traynor-Kaplan A, Schultz C, Erbay E. Inositol-requiring enzyme-1 regulates phosphoinositide signaling lipids and macrophage growth. (2020) EMBO Rep. Dec 3;21(12):e51462. doi: 10.15252/embr.202051462. Epub 2020 Nov 2. PMID: 33140520; PMCID: PMC7726810.
- Basappa J, Citir M, Zhang Q, Wang HY, Liu X, Melnikov O, Yahya H, Stein F, Muller R, Traynor-Kaplan A, Schultz C, Wasik MA, Ptasznik A. ACLY Is the Novel Signaling Target of PIP 2/PIP3 and Lyn in Acute Myeloid Leukemia . (2020) Heliyon May 7;6(5):e03910. doi: 10.1016/j.heliyon.2020.e03910. eCollection 2020 May.
- de la Cruz, L., Traynor-Kaplan, A.,Vivas, O., Hille, B., Jensen, JB, Plasma membrane processes differentially regulated by type I phosphatidylinositol phosphate 5-kinases and RASSF4 (2019) Journal of Cell Science, 12 December: jcs.233254 doi: 10.1242/jcs.233254
- Mücksch, F., Citir, M., Lüchtenborg, C., Glass, B., Traynor-Kaplan, A., Schultz, C., Brügger, B. Kräusslich, H.G. . Quantification of phosphoinositides reveals strong enrichment of PIP2 in HIV-1 compared to producer cell membranes. (2019) Sci Rep 9, 17661 doi:10.1038/s41598-019-53939-z. https://www.ncbi.nlm.nih.gov/pubmed/31776383
- So, V., Wu, J., Traynor-Kaplan, A.,Choy, C.,Epand, R., Botelho, R., Lemaire, M. Phosphatidylinositol Cycle Disruption is Central to Atypical Hemolytic-Uremic Syndrome Caused by Diacylglycerol Kinase Epsilon Deficiency. (2019) bioRxiv 633867; doi: https://doi.org/10.1101/633867
- Traynor-Kaplan A, Kruse M, Dickson EJ, Dai G, Vivas O, Yu H, Whittington D, Hille B. Fatty-acyl chain profiles of cellular phosphoinositides. (2017) Biochim Biophys Acta. Feb 9;1862(5):513-522. doi: 10.1016/j.bbalip.2017.02.002 https://www.ncbi.nlm.nih.gov/pubmed/28189644
- Kruse, M., O. Vivas, A. Traynor-Kaplan, and B. Hille. . Dynamics of Phosphoinositide-Dependent Signaling in Sympathetic Neurons. (2016) J Neurosci 36: 1386-1400. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4728732/
- Dai, G., H. Yu, M. Kruse, A. Traynor-Kaplan, and B. Hille. Osmoregulatory inositol transporter SMIT1 modulates electrical activity by adjusting PI(4,5)P2 levels. (2016) Proc Natl Acad Sci U S A 113: E3290-3299. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4988571/
- Dickson, E. J., J. B. Jensen, O. Vivas, M. Kruse, A. E. Traynor-Kaplan, and B. Hille. Dynamic formation of ER-PM junctions presents a lipid phosphatase to regulate phosphoinositides. (2016) J Cell Biol 213: 33-48. https://www.ncbi.nlm.nih.gov/pubmed/27044890
- V. Laketa, S. Zarbakhsh, A. Traynor-Kaplan, A. MacNamara, D. Subramanian, M. Putyrski, R. Mueller, A. Nadler, M. Mentel, J. Saez-Rodriguez, R. Pepperkok, C. Schultz, PIP3 Induces the Recycling of Receptor Tyrosine Kinases. (2014). Sci. Signal. 7, ra5 https://www.ncbi.nlm.nih.gov/pubmed/24425787
Recent Client Publications
Horvath JD, Casas M, Kutchukian C, Sánchez SC, Pergande MR, Cologna SM, Simó S, Dixon RE, Dickson EJ. α-Synuclein-dependent increases in PIP5K1γ drive inositol signaling to promote neurotoxicity. Cell Rep. 2023 Oct 31;42(10):113244. doi: 10.1016/j.celrep.2023.113244. Epub 2023 Oct 14. PMID: 37838947.
de la Cruz L, Kushmerick C, Sullivan JM, Kruse M, Vivas O. Hippocampal neurons maintain a large PtdIns(4)P pool that results in faster PtdIns(4,5)P2 synthesis. J Gen Physiol. 2022 Mar 7;154(3):e202113001. doi: 10.1085/jgp.202113001. Epub 2022 Feb 18. PMID: 35179558; PMCID: PMC8906353.
de la Cruz L, Riquelme R, Vivas O, Barria A, Jensen JB. Dishevelled coordinates phosphoinositide kinases PI4KIIIα and PIP5KIγ for efficient PtdInsP2 synthesis. J Cell Sci. 2022 Mar 1;135(5):jcs259145. doi: 10.1242/jcs.259145. Epub 2022 Feb 3. PMID: 34982154; PMCID: PMC8919331.
Myeong J, de la Cruz L, Jung SR, Yeon JH, Suh BC, Koh DS, Hille B. Phosphatidylinositol 4,5-bisphosphate is regenerated by speeding of the PI 4-kinase pathway during long PLC activation. J Gen Physiol. 2020 Dec 7;152(12):e202012627. doi: 10.1085/jgp.202012627. PMID: 33186442; PMCID: PMC7671494.
Verdura E, Rodríguez-Palmero A, Vélez-Santamaria V, Planas-Serra L, de la Calle I, Raspall-Chaure M, Roubertie A, Benkirane M, Saettini F, Pavinato L, Mandrile G, O'Leary M, O'Heir E, Barredo E, Chacón A, Michaud V, Goizet C, Ruiz M, Schlüter A, Rouvet I, Sala-Coromina J, Fossati C, Iascone M, Canonico F, Marcé-Grau A, de Souza P, Adams DR, Casasnovas C, Rehm HL, Mefford HC, González Gutierrez-Solana L, Brusco A, Koenig M, Macaya A, Pujol A. Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy. Brain. 2021 Oct 22;144(9):2659-2669. doi: 10.1093/brain/awab124. PMID: 34415322; PMCID: PMC8557332.
Saliakoura, M., Reynoso-Moreno, I., Pozzato, C., Rossi Sebastiano, M., Galié, M., Gertsch, J., & Konstantinidou, G. The ACSL3-LPIAT1 signaling drives prostaglandin synthesis in non-small cell lung cancer. Oncogene, 2020 39(14), 2948–2960. https://doi.org/10.1038/s41388-020-1196-5
Vivas O, Tiscione SA, Dixon RE, Ory DS, Dickson EJ. Niemann-Pick Type C Disease Reveals a Link between Lysosomal Cholesterol and PtdIns(4,5)P2 That Regulates Neuronal Excitability. Cell Rep. 2019 May 28;27(9):2636-2648.e4. doi: 10.1016/j.celrep.2019.04.099. PMID: 31141688; PMCID: PMC6553496.
Botero S, Chiaroni-Clarke R, Simon SM. Escherichia coli as a platform for the study of phosphoinositide biology. Sci Adv. 2019;5(3):eaat4872. Published 2019 Mar 27. doi:10.1126/sciadv.aat4872 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436935/
Alvarez-Prats A, Bjelobaba I, Aldworth Z, Baba T, Abebe D, Kim YJ, Stojilkovic SS, Stopfer M, Balla T. Schwann-Cell-Specific Deletion of Phosphatidylinositol 4-Kinase Alpha Causes Aberrant Myelination. Cell Rep. 2018 Jun 5;23(10):2881-2890. doi: 10.1016/j.celrep.2018.05.019. PMID: 29874576; PMCID: PMC7268203.
Horvath JD, Casas M, Kutchukian C, Sánchez SC, Pergande MR, Cologna SM, Simó S, Dixon RE, Dickson EJ. α-Synuclein-dependent increases in PIP5K1γ drive inositol signaling to promote neurotoxicity. Cell Rep. 2023 Oct 31;42(10):113244. doi: 10.1016/j.celrep.2023.113244. Epub 2023 Oct 14. PMID: 37838947.
de la Cruz L, Kushmerick C, Sullivan JM, Kruse M, Vivas O. Hippocampal neurons maintain a large PtdIns(4)P pool that results in faster PtdIns(4,5)P2 synthesis. J Gen Physiol. 2022 Mar 7;154(3):e202113001. doi: 10.1085/jgp.202113001. Epub 2022 Feb 18. PMID: 35179558; PMCID: PMC8906353.
de la Cruz L, Riquelme R, Vivas O, Barria A, Jensen JB. Dishevelled coordinates phosphoinositide kinases PI4KIIIα and PIP5KIγ for efficient PtdInsP2 synthesis. J Cell Sci. 2022 Mar 1;135(5):jcs259145. doi: 10.1242/jcs.259145. Epub 2022 Feb 3. PMID: 34982154; PMCID: PMC8919331.
Myeong J, de la Cruz L, Jung SR, Yeon JH, Suh BC, Koh DS, Hille B. Phosphatidylinositol 4,5-bisphosphate is regenerated by speeding of the PI 4-kinase pathway during long PLC activation. J Gen Physiol. 2020 Dec 7;152(12):e202012627. doi: 10.1085/jgp.202012627. PMID: 33186442; PMCID: PMC7671494.
Verdura E, Rodríguez-Palmero A, Vélez-Santamaria V, Planas-Serra L, de la Calle I, Raspall-Chaure M, Roubertie A, Benkirane M, Saettini F, Pavinato L, Mandrile G, O'Leary M, O'Heir E, Barredo E, Chacón A, Michaud V, Goizet C, Ruiz M, Schlüter A, Rouvet I, Sala-Coromina J, Fossati C, Iascone M, Canonico F, Marcé-Grau A, de Souza P, Adams DR, Casasnovas C, Rehm HL, Mefford HC, González Gutierrez-Solana L, Brusco A, Koenig M, Macaya A, Pujol A. Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy. Brain. 2021 Oct 22;144(9):2659-2669. doi: 10.1093/brain/awab124. PMID: 34415322; PMCID: PMC8557332.
Saliakoura, M., Reynoso-Moreno, I., Pozzato, C., Rossi Sebastiano, M., Galié, M., Gertsch, J., & Konstantinidou, G. The ACSL3-LPIAT1 signaling drives prostaglandin synthesis in non-small cell lung cancer. Oncogene, 2020 39(14), 2948–2960. https://doi.org/10.1038/s41388-020-1196-5
Vivas O, Tiscione SA, Dixon RE, Ory DS, Dickson EJ. Niemann-Pick Type C Disease Reveals a Link between Lysosomal Cholesterol and PtdIns(4,5)P2 That Regulates Neuronal Excitability. Cell Rep. 2019 May 28;27(9):2636-2648.e4. doi: 10.1016/j.celrep.2019.04.099. PMID: 31141688; PMCID: PMC6553496.
Botero S, Chiaroni-Clarke R, Simon SM. Escherichia coli as a platform for the study of phosphoinositide biology. Sci Adv. 2019;5(3):eaat4872. Published 2019 Mar 27. doi:10.1126/sciadv.aat4872 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436935/
Alvarez-Prats A, Bjelobaba I, Aldworth Z, Baba T, Abebe D, Kim YJ, Stojilkovic SS, Stopfer M, Balla T. Schwann-Cell-Specific Deletion of Phosphatidylinositol 4-Kinase Alpha Causes Aberrant Myelination. Cell Rep. 2018 Jun 5;23(10):2881-2890. doi: 10.1016/j.celrep.2018.05.019. PMID: 29874576; PMCID: PMC7268203.